anti-CD25 antibody [OX-39] (PE)
![anti-CD25 antibody [OX-39] (PE)](/upload/image/products/ARG23024_FACS_1_210_205.jpg)
![anti-CD25 antibody [OX-39] (PE)](/upload/image/products/ARG23024_FACS_1.jpg)
Key features and details
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- 克隆:
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- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG23024
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [OX-39] recognizes CD25 Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987). |
|---|---|
| 反应物种 | Rat |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | OX-39 |
| 同位型 | IgG1 |
| 靶点名称 | CD25 |
| 抗原物种 | Rat |
| 抗原 | Stimulated Rat T cells |
| 偶联标记 | PE |
| 別名 | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P26897 Rat Interleukin-2 receptor subunit alpha |
|---|---|
| 基因名称 | Il2ra |
| 全名 | interleukin 2 receptor, alpha |
| 背景介绍 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
| 生物功能 | Receptor for interleukin-2. [UniProt] |
| 研究领域 | Immune System antibody; Pre-B Cell Marker antibody |
| 预测分子量 | 31 kDa |
检测图片 (1)
ARG23024 anti-CD25 antibody [OX-39] (PE) FACS image
Flow Cytometry: ConA activated rat splenic lymphocytes stained with ARG23024 anti-CD25 antibody [OX-39] (PE).
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