anti-CD28 antibody [CD28.2] (low endotoxin)
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Key features and details
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Product Details
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [CD28.2] recognizes CD28 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB |
| 特异性 | The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. HLDA V.; WS Code 5T CD28.05 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CD28.2 |
| 同位型 | IgG1 |
| 靶点名称 | CD28 |
| 抗原物种 | Human |
| 抗原 | DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA |
| 偶联标记 | Un-conjugated |
| 別名 | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
| 应用建议 |
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| 应用说明 | Functional studies: T cell costimulation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28 |
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| 基因名称 | CD28 |
| 全名 | CD28 molecule |
| 背景介绍 | CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. |
| 生物功能 | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | CD40LG induces tyrosine phosphorylation of isoform 3. |
ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) at 1.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) FACS image
Flow Cytometry: Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65354 anti-CD28 antibody [CD28.2] (low endotoxin) at 1.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
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