anti-CD28 antibody [CD28.2] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [CD28.2] recognizes CD28
反应物种: Hu, NHuPrm
应用: FACS
特异性: The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.HLDA V.; WS Code 5T CD28.05
宿主: Mouse
克隆: Monoclonal
克隆号: CD28.2
同位型: IgG1
靶点名称: CD28

CAT.NO. : ARG54233

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Size:

50 tests

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [CD28.2] recognizes CD28
反应物种	Hu, NHuPrm
应用	FACS
特异性	The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. HLDA V.; WS Code 5T CD28.05
宿主	Mouse
克隆	Monoclonal
克隆号	CD28.2
同位型	IgG1
靶点名称	CD28
抗原物种	Human
抗原	DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
偶联标记	PE
別名	Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 940 Human CD28 Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28
基因名称	CD28
全名	CD28 molecule
背景介绍	CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
生物功能	Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
预测分子量	25 kDa
翻译后修饰	CD40LG induces tyrosine phosphorylation of isoform 3.

检测图片 (1)

ARG54233 anti-CD28 antibody [CD28.2] (PE) FACS image
Flow Cytometry: Human peripheral blood leukocytes stained with ARG54233 anti-CD28 antibody [CD28.2] (PE).

参考文献

克隆号文献

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

FuncSt / Human

Chen ML et al.
Immunol Lett., (2011)

NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis.

Urso K et al.
Blood., (2011)

Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.

Jeong SH et al.
J Virol., (2004)

Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling.

Scharschmidt E et al.
Mol Cell Biol., (2004)

Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.

Marti F et al.
J Immunol., (2001)

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