anti-CD3 antibody [SP162]

Key features and details

产品描述: Rabbit Monoclonal antibody [SP162] recognizes CD3
反应物种: Hu
预测物种: Ms, Rat, Bov, Chk, Dog, Pig, Rb
应用: FACS, IHC-P, WB
宿主: Rabbit
克隆: Monoclonal
克隆号: SP162
同位型: IgG
靶点名称: CD3

Brand:

CAT.NO. : ARG52742

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Size:

100 μl 50 μl

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Rabbit Monoclonal antibody [SP162] recognizes CD3
反应物种	Hu
预测物种	Ms, Rat, Bov, Chk, Dog, Pig, Rb
应用	FACS, IHC-P, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SP162
同位型	IgG
靶点名称	CD3
抗原物种	Human
抗原	Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein.
偶联标记	Un-conjugated
別名	T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-Dependent
IHC-P	1:150
WB	1:150

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

Tonsil, Jurkat

属性

形式	Liquid
纯化	Purified by protein A/G
缓冲液	PBS (pH 7.6), 1% BSA and
抗菌剂
稳定剂	1% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 916 Human CD3E Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain
背景介绍	CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
生物功能	CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
细胞定位	Membrane
产品亮点	Related Antibody Duos and Panels: ARG30127 Lymphocyte Marker Antibody Duo (CD3, CD19)(IHC/ICC) ARG30313 General Lymphocyte Marker Antibody Panel (CD3, CD14, CD16, CD19, CD56) Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response
研究领域	Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
预测分子量	23 kDa

检测图片 (12)

ARG52742 anti-CD3 antibody [SP162] WB image
Western Blot: Jurkate Cell Lysate stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Fallopian Tube stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human B-cell lymphoma stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Thymus stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human HK lymphoma stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Cervix stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Spleen stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Esophagus stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Colon stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Bladder stained with CD3 antibody [SP162] (ARG52742)
ARG52742 anti-CD3 antibody [SP162] IHC-P image
Immunohistochemistry: Human Liver stained with CD3 antibody [SP162] (ARG52742)