anti-CD32B antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes CD32B
  • 反应物种: Hu
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CD32B
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63774
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes CD32B
反应物种Hu
应用IHC-P, WB
特异性This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称CD32B
抗原物种Human
抗原C-PDALEEPDDQNRI
偶联标记Un-conjugated
別名Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32
应用说明
应用建议
应用推荐稀释比
IHC-PAssay - dependent
WB0.3 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2213 Human FCGR2B

Swiss-port # P31994 Human Low affinity immunoglobulin gamma Fc region receptor II-b

背景介绍The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
研究领域Developmental Biology antibody; Immune System antibody
预测分子量36 kDa
翻译后修饰Phosphorylated by the SRC-type Tyr-kinases LYN and BLK.
检测图片 (5)
  • ARG63774 anti-CD32B antibody WB image

    Western blot: K562 lysate (35 µg protein in RIPA buffer) stained with ARG63774 anti-CD32B antibody at 0.3 µg/ml dilution.

  • ARG63774 anti-CD32B antibody WB image

    Western blot: 1). Mock transfection; 2) CD32 (RC211982) expressing plasmid transfected HEK293 cell lysate standed with ARG63774 anti-CD32B antibody.

  • ARG63774 anti-CD32B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63774 anti-CD32B antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63774 anti-CD32B antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63774 anti-CD32B antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG63774 anti-CD32B antibody WB image

    Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG63774 anti-CD32B antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

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