anti-CD34 antibody [4H11(APG)]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [4H11(APG)] recognizes CD34
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 特异性: The clone 4H11(APG) reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. 4H11(APG) completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.HLDA VI; WS Code M MA58
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4H11(APG)
  • 同位型: IgG1
  • 靶点名称: CD34
  • Brand:
CAT.NO. : ARG62820
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Product Details
概述
产品描述Mouse Monoclonal antibody [4H11(APG)] recognizes CD34
反应物种Hu
应用FACS, ICC/IF, IHC-P, WB
特异性The clone 4H11(APG) reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. 4H11(APG) completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
HLDA VI; WS Code M MA58
宿主Mouse
克隆Monoclonal
克隆号4H11(APG)
同位型IgG1
靶点名称CD34
抗原物种Human
抗原Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
偶联标记Un-conjugated
別名Hematopoietic progenitor cell antigen CD34; CD antigen CD34
应用说明
应用建议
应用推荐稀释比
FACS2 µg/ml
ICC/IFAssay-dependent
IHC-P10 µg/ml
WB1 - 2 µg/ml
应用说明WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照WB: Positive control: Kg-1a human leukemia cells. Negative control: Jurkat.
IHC-P: Positive control: Placenta endothelium.
属性
形式Liquid
纯化Purified from ascites by precipitation methods.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

基因名称CD34
全名CD34 molecule
背景介绍CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
生物功能CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
产品亮点Related Antibody Duos and Panels:
ARG30306 Pro-B Cell Marker Antibody panel (CD19, CD34, CD38, CD40, CD45) (FACS)
Related products:
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies;
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
预测分子量41 kDa
翻译后修饰Highly glycosylated.
Phosphorylated on serine residues by PKC.
检测图片 (1)
  • ARG62820 anti-CD34 antibody [4H11(APG)] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG62820 anti-CD34 antibody [4H11(APG)], followed by incubation with APC labelled Goat anti-Mouse secondary antibody. CD34+ cells (red); CD45+ cells (black).

参考文献

克隆号文献

A novel and feasible way to cultivate and purify endothelial progenitor cells from bone marrow of children with congenital heart diseases.

FACS / Human

Wu YT et al.
Chin Med J (Engl).,  (2012)

publication_link

 

hr_line

5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype.

FACS / Human

Castro FV et al.
Leukemia.,  (2012)

publication_link

 

hr_line

Growth factor priming of synovium-derived stem cells for cartilage tissue engineering.

FACS / Cow

Sampat SR et al.
Tissue Eng Part A.,  (2011)

publication_link

 

hr_line

Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs).

FACS / Human

Gong Z et al.
FASEB J.,  (2008)

publication_link

 

hr_line
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