anti-CD42b antibody [HIP1]
![anti-CD42b antibody [HIP1]](/upload/image/products/ARG62846_FACS_2_201117_210_205.jpg)
![anti-CD42b antibody [HIP1]](/upload/image/products/ARG62846_FACS_1_201117.jpg)
![anti-CD42b antibody [HIP1]](/upload/image/products/ARG62846_FACS_2_201117.jpg)
Key features and details
- 产品描述:
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- 应用:
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- 靶点名称:
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CAT.NO. : ARG62846
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [HIP1] recognizes CD42b |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | CyTOF®-candidate, FACS, FuncSt, IHC-Fr |
| 特异性 | The clone HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HIP1 |
| 同位型 | IgG1 |
| 靶点名称 | CD42b |
| 抗原 | Peripheral blood mononuclear cells of a patient suffering with CLL. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD42b; Antigen CD42b-alpha; DBPLT3; VWDP; CD42B; GP-Ib alpha; Glycoprotein Ibalpha; BDPLT1; BSS; CD42b-alpha; GPIbA; GPIb-alpha; Platelet glycoprotein Ib alpha chain; BDPLT3; GP1B |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Functional studies: The clone HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P07359 Human Platelet glycoprotein Ib alpha chain |
|---|---|
| 基因名称 | GP1BA |
| 全名 | glycoprotein Ib (platelet), alpha polypeptide |
| 背景介绍 | CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. |
| 生物功能 | GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. [UniProt] |
| 产品亮点 | Related products: CD42b antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody |
| 预测分子量 | 72 kDa |
| 翻译后修饰 | Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. |
检测图片 (2)
ARG62846 anti-CD42b antibody [HIP1] FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG62846 anti-CD42b antibody [HIP1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62846 anti-CD42b antibody [HIP1] FACS image
Flow Cytometry: Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62846 anti-CD42b antibody [HIP1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
参考文献
克隆号文献
Streptococcus mitis phage-encoded adhesins mediate attachment to {alpha}2-8-linked sialic acid residues on platelet membrane gangliosides.
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