anti-CD43 antibody [84-3C1]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [84-3C1] recognizes CD43
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 84-3C1
  • 同位型: IgG1, kappa
  • 靶点名称: CD43
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56077
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Product Details
概述
产品描述Mouse Monoclonal antibody [84-3C1] recognizes CD43
反应物种Hu
应用FACS, ICC/IF, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号84-3C1
同位型IgG1, kappa
靶点名称CD43
抗原物种Human
抗原Stimulated Human leukocytes.
偶联标记Un-conjugated
別名LSN; CD43; GALGP; GPL115
应用说明
应用建议
应用推荐稀释比
FACS0.5 - 1 µg/10^6 cells in 0.1ml
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 1 µg/ml
WB1 - 2 µg/ml
应用说明Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂0.05% Sodium azide
稳定剂0.1 mg/ml BSA
浓度0.2 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6693 Human SPN

Swiss-port # P16150 Human Leukosialin

基因名称SPN
全名sialophorin
背景介绍The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]
生物功能One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]
细胞定位Cell surface
预测分子量40 kDa
检测图片 (2)
  • ARG56077 anti-CD43 antibody [84-3C1] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56077 anti-CD43 antibody [84-3C1].

  • ARG56077 anti-CD43 antibody [84-3C1] WB image

    Western blot: Ramos cell lysate stained with ARG56077 anti-CD43 antibody [84-3C1].

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