anti-CD50 / ICAM3 antibody [MEM-171] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [MEM-171] recognizes CD50 / ICAM3
反应物种: Hu
应用: FACS
特异性: The clone MEM-171 recognizes an epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. HLDA VI; WS Code BP 614 HLDA VI; WS Code NL N-L022
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-171
同位型: IgG1
靶点名称: CD50 / ICAM3

CAT.NO. : ARG53869

US＄ Please choose

Size:

100 tests

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [MEM-171] recognizes CD50 / ICAM3
反应物种	Hu
应用	FACS
特异性	The clone MEM-171 recognizes an epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. HLDA VI; WS Code BP 614 HLDA VI; WS Code NL N-L022
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-171
同位型	IgG1
靶点名称	CD50 / ICAM3
抗原物种	Human
抗原	Human granulocytes
偶联标记	PE
別名	CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3385 Human ICAM3 Swiss-port # P32942 Human Intercellular adhesion molecule 3
基因名称	ICAM3
全名	intercellular adhesion molecule 3
背景介绍	CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specificICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
生物功能	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt]
研究领域	Immune System antibody; Signaling Transduction antibody
预测分子量	60 kDa
翻译后修饰	Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

检测图片 (1)

ARG53869 anti-CD50 / ICAM3 antibody [MEM-171] (PE) FACS image
Flow Cytometry: Human peripheral blood stained with ARG53869 anti-CD50 / ICAM3 antibody [MEM-171] (PE).

参考文献

克隆号文献

Lymphocytes can self-steer passively with wind vane uropods.

Valignat MP et al.
Nat Commun., (2014)