anti-CD53 antibody [MEM-53] (FITC)

产品描述: FITC-conjugated Mouse Monoclonal antibody [MEM-53] recognizes CD53
反应物种: Hu
应用: FACS
特异性: The clone MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).HLDA IV; WS Code NL 59HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-53
同位型: IgG1
靶点名称: CD53

CAT.NO. : ARG62884

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Size:

100 tests

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [MEM-53] recognizes CD53
反应物种	Hu
应用	FACS
特异性	The clone MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-53
同位型	IgG1
靶点名称	CD53
抗原	Leukocytes of pacient suffering from a LGL-type leukemia.
偶联标记	FITC
別名	Tetraspanin-25; Leukocyte surface antigen CD53; Tspan-25; Cell surface glycoprotein CD53; CD antigen CD53; MOX44; TSPAN25

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 963 Human CD53 Swiss-port # P19397 Human Leukocyte surface antigen CD53
基因名称	CD53
全名	CD53 molecule
背景介绍	CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
生物功能	Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). [UniProt]
研究领域	Immune System antibody; Signaling Transduction antibody
预测分子量	24 kDa

检测图片 (1)

ARG62884 anti-CD53 antibody [MEM-53] (FITC) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG62884 anti-CD53 antibody [MEM-53] (FITC).

参考文献

克隆号文献

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol., (2012)