anti-CD54 / ICAM1 antibody [84H10]
![anti-CD54 / ICAM1 antibody [84H10]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
Key features and details
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- 克隆:
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- 抗原物种:
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CAT.NO. : ARG23061
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [84H10] recognizes CD54 / ICAM1 Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD.Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. |
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| 反应物种 | Hu, Dog |
| 应用 | ELISA, FACS, FuncSt, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 84H10 |
| 同位型 | IgG1 |
| 靶点名称 | CD54 / ICAM1 |
| 抗原物种 | Human |
| 抗原 | K562 cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
应用说明
| 应用建议 |
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| 应用说明 | Functional study: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
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| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ICAM1 |
| 全名 | intercellular adhesion molecule 1 |
| 背景介绍 | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| 生物功能 | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
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