anti-CD64 antibody [10.1]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [10.1] recognizes CD64
  • 反应物种: Hu, NHuPrm
  • 应用: CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB
  • 特异性: The clone 10.1 recognizes alpha subunit of CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.HLDA III; WS Code M-250
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 10.1
  • 同位型: IgG1
  • 靶点名称: CD64
  • Brand:
CAT.NO. : ARG65361
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Product Details
概述
产品描述Mouse Monoclonal antibody [10.1] recognizes CD64
反应物种Hu, NHuPrm
应用CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性The clone 10.1 recognizes alpha subunit of CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes.
HLDA III; WS Code M-250
宿主Mouse
克隆Monoclonal
克隆号10.1
同位型IgG1
靶点名称CD64
抗原物种Human
抗原Rheumatoid synovial fluid cells and fibronectin purified human monocytes
偶联标记Un-conjugated
別名High affinity immunoglobulin gamma Fc receptor I; CD64; Fc-gamma RIA; CD antigen CD64; FcgammaRIa; FCRI; IgG Fc receptor I; CD64A; Fc-gamma RI; FcRI; IGFR1
应用说明
应用建议
应用推荐稀释比
CyTOF®-candidateAssay-dependent
FACS1 - 5 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
应用说明IHC-P and IHC-Fr: There can occur problems with paraformaldehyde fixation
Functional studies: blocking of IgG binding to the FcgammaR1.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from cell culture supernatant by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2209 Human FCGR1A

Swiss-port # P12314 Human High affinity immunoglobulin gamma Fc receptor I

基因名称FCGR1A
全名Fc fragment of IgG, high affinity Ia, receptor (CD64)
背景介绍CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
生物功能High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. [UniProt]
产品亮点Related products:
CD64 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
研究领域Immune System antibody
预测分子量43 kDa
翻译后修饰Phosphorylated on serine residues.
检测图片 (2)
  • ARG65361 anti-CD64 antibody [10.1] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG65361 anti-CD64 antibody [10.1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

  • ARG65361 anti-CD64 antibody [10.1] FACS image

    Flow Cytometry: Separation of human monocytes (red-filled) from CD64 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65361 anti-CD64 antibody [10.1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

参考文献

克隆号文献

Rap1 activation is required for Fc gamma receptor-dependent phagocytosis.

FACS / 

Chung J et al.
J Immunol.,  (2008)

publication_link

 

hr_line

CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells.

Sánchez-Torres C et al.
Int Immunol.,  (2001)

publication_link

 

hr_line

Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

Yoshino N et al.
Exp Anim.,  (2000)

publication_link

 

hr_line

High affinity IgG binding by FcgammaRI (CD64) is modulated by two distinct IgSF domains and the transmembrane domain of the receptor.

Harrison PT et al.
Protein Eng.,  (1998)

publication_link

 

hr_line
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