anti-CD71 / Transferrin Receptor antibody [DF1513]

CAT.NO. : ARG56159

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Size:

50 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [DF1513] recognizes CD71 / Transferrin Receptor
反应物种	Hu
应用	FACS, ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	DF1513
同位型	IgG1, kappa
靶点名称	CD71 / Transferrin Receptor
抗原物种	Human
抗原	KG1 acute myeloid leukemia cell line.
偶联标记	Un-conjugated
別名	TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
抗菌剂	0.05% Sodium azide
稳定剂	0.1 mg/ml BSA
浓度	0.2 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 7037 Human TFRC Swiss-port # P02786 Human Transferrin receptor protein 1
基因名称	TFRC
全名	transferrin receptor
背景介绍	This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
生物功能	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt]
预测分子量	~ 85 - 95 kDa
翻译后修饰	N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

检测图片 (2)

ARG56159 anti-CD71 / Transferrin Receptor antibody [DF1513] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG56159 anti-CD71 / Transferrin Receptor antibody [DF1513] at 1:100 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG56159 anti-CD71 / Transferrin Receptor antibody [DF1513] WB image
Western blot: 30 µg of HeLa cell lysates stained with ARG56159 anti-CD71 / Transferrin Receptor antibody [DF1513] at 0.25 µg/ml dilution.

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