anti-CD73 antibody [2B6]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [2B6] recognizes CD73
  • 反应物种: Hu
  • 应用: FACS, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 2B6
  • 同位型: IgG2b, kappa
  • 靶点名称: CD73
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56944
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Product Details
概述
产品描述Mouse Monoclonal antibody [2B6] recognizes CD73
反应物种Hu
应用FACS, WB
宿主Mouse
克隆Monoclonal
克隆号2B6
同位型IgG2b, kappa
靶点名称CD73
抗原物种Human
抗原Recombinant fragment around aa. 27-252 of Human CD73.
偶联标记Un-conjugated
別名5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE
应用说明
应用建议
应用推荐稀释比
FACSAssay-dependent
WB1:1000 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

基因名称NT5E
全名5'-nucleotidase, ecto (CD73)
背景介绍The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
生物功能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
预测分子量63 kDa
检测图片 (3)
  • ARG56944 anti-CD73 antibody [2B6] FACS image

    Flow Cytometry: PBMC cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG56944 anti-CD73 antibody [2B6] WB image

    Western blot: 30 µg of Human mesenchymal stem cell lysate stained with ARG56944 anti-CD73 antibody [2B6] at 1:1000.

  • ARG56944 anti-CD73 antibody [2B6] FACS image

    Flow Cytometry: LNCaP cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line ).

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