anti-CD86 antibody [24F] (PE)
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Key features and details
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- 反应物种:
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- 克隆:
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- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG23463
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [24F] recognizes CD86 Mouse anti Rat CD86 antibody, clone 24F recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maeda et al. 1997). |
|---|---|
| 反应物种 | Rat |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 24F |
| 同位型 | IgG1 |
| 靶点名称 | CD86 |
| 抗原物种 | Rat |
| 抗原 | HTLV-1 transformed Lewis-S1 cells. |
| 偶联标记 | PE |
| 別名 | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CD86 |
|---|---|
| 全名 | CD86 molecule |
| 背景介绍 | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| 生物功能 | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt] |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt] |
检测图片 (2)
ARG23463 anti-CD86 antibody [24F] (PE) FACS image
Flow Cytometry: Figure A. A488 conjugated Mouse anti Rat CD45 and PE-conjugated Mouse IgG1 isotype control. Figure B. A488 conjugated Mouse anti Rat CD45 and ARG23463 anti-CD86 antibody [24F] (PE). All experiments performed on red cell lysed Rat blood gated on mononuclear cells.
ARG23463 anti-CD86 antibody [24F] (PE) FACS image
Flow Cytometry: Figure A. ARG23463 anti-CD86 antibody [24F] (PE) and A488 conjugated Mouse IgG1 isotype control. Figure B. ARG23463 anti-CD86 antibody [24F] (PE) and A488 conjugated Mouse anti Rat CD45. All experiments performed on red cell lysed Rat blood gated on mononuclear cells.
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