anti-CD86 antibody [BU63] (low endotoxin)
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Key features and details
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- 靶点名称:
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Brand:
CAT.NO. : ARG42258
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Product Details
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [BU63] recognizes CD86 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-Fr, IP, WB |
| 特异性 | The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BU63 |
| 同位型 | IgG1 |
| 靶点名称 | CD86 |
| 抗原物种 | Human |
| 抗原 | B-lymphoblastoid cell line ARH-77. |
| 偶联标记 | Un-conjugated |
| 別名 | B70; B7.2; LAB72; CD antigen CD86; B7-2; FUN-1; CD28LG2; T-lymphocyte activation antigen CD86; CTLA-4 counter-receptor B7.2; Activation B7-2 antigen; BU63 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P42081 Human T-lymphocyte activation antigen CD86 |
|---|---|
| 基因名称 | CD86 |
| 全名 | CD86 molecule |
| 背景介绍 | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, May 2011] |
| 生物功能 | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. [UniProt] |
检测图片 (2)
ARG42258 anti-CD86 antibody [BU63] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42258 anti-CD86 antibody [BU63] (low endotoxin) at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG42258 anti-CD86 antibody [BU63] (low endotoxin) FACS image
Flow Cytometry: Separation of Human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42258 anti-CD86 antibody [BU63] (low endotoxin) at 3 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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