anti-CD90 / Thy 1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CD90 / Thy 1
  • 反应物种: Ms, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CD90 / Thy 1
  • 抗原物种: Mouse
  • 抗原: Recombinant protein corresponding to Q20-C131 of Mouse CD90 / Thy 1.
  • Brand:
CAT.NO. : ARG41919
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CD90 / Thy 1
反应物种Ms, Rat
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CD90 / Thy 1
抗原物种Mouse
抗原Recombinant protein corresponding to Q20-C131 of Mouse CD90 / Thy 1.
偶联标记Un-conjugated
別名Thy-1 membrane glycoprotein; Thy-1 antigen; CD antigen CD90; CDw90; CD90
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量~ 27 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 21838 Mouse THY1

GeneID: 24832 Rat THY1

Swiss-port # P01830 Rat Thy-1 membrane glycoprotein

Swiss-port # P01831 Mouse Thy-1 membrane glycoprotein

基因名称THY1
全名Thy-1 cell surface antigen
背景介绍This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
生物功能May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. [UniProt]
细胞定位Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
预测分子量18 kDa
检测图片 (5)
  • ARG41919 anti-CD90 / Thy 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41919 anti-CD90 / Thy 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG41919 anti-CD90 / Thy 1 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Mouse brain, Mouse thymus, Mouse lung and Rat thymus lysates stained with ARG41919 anti-CD90 / Thy 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG41919 anti-CD90 / Thy 1 antibody FACS image

    Flow Cytometry: YAC-1 cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG41919 anti-CD90 / Thy 1 antibody FACS image

    Flow Cytometry: Neuro-2a cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG41919 anti-CD90 / Thy 1 antibody FACS image

    Flow Cytometry: SP2-0 cells were blocked with 10% normal goat serum and then stained with ARG41919 anti-CD90 / Thy 1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

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