anti-CD99 antibody [3B2/TA8] (FITC)

产品描述: FITC-conjugated Mouse Monoclonal antibody [3B2/TA8] recognizes CD99
反应物种: Hu
应用: FACS
特异性: The clone 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes. HLDA VI.; WS Code T 6T-097, BP 534
宿主: Mouse
克隆: Monoclonal
克隆号: 3B2/TA8
同位型: IgG2a
靶点名称: CD99

CAT.NO. : ARG65390

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Size:

50 tests

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Product Details

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [3B2/TA8] recognizes CD99
反应物种	Hu
应用	FACS
特异性	The clone 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes. HLDA VI.; WS Code T 6T-097, BP 534
宿主	Mouse
克隆	Monoclonal
克隆号	3B2/TA8
同位型	IgG2a
靶点名称	CD99
抗原物种	Human
抗原	Human thymocytes
偶联标记	FITC
別名	12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

应用说明

应用建议

应用	推荐稀释比
FACS	4 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4267 Human CD99 Swiss-port # P14209 Human CD99 antigen
基因名称	CD99
全名	CD99 molecule
背景介绍	CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
生物功能	CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
研究领域	Cancer antibody; Immune System antibody; Signaling Transduction antibody
预测分子量	19 kDa
翻译后修饰	Extensively O-glycosylated.

检测图片 (2)

ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG65390 anti-CD99 antibody [3B2/TA8] (FITC).
ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) FACS image
Flow Cytometry: Separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65390 anti-CD99 antibody [3B2/TA8] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).