anti-CDK1 / CDC2 phospho (Tyr15) antibody
Key features and details
- 产品描述:
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- 预测物种:
- 应用:
- 宿主:
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- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52239
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDK1 / CDC2 phospho (Tyr15) |
|---|---|
| 反应物种 | Hu, Xenopus laevis |
| 预测物种 | Ms, Rat, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDK1 / CDC2 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | CDK1; Cyclin Dependent Kinase 1; CDC28A; CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDK1 |
| 全名 | cyclin-dependent kinase 1 |
| 背景介绍 | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2023] |
| 生物功能 | Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins. [UniProt] |
| 细胞定位 | Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint. [UniProt] |
检测图片 (1)
ARG52239 anti-CDK1 / CDC2 phospho (Tyr15) antibody WB image
Western blot: Human T47D cells showing phospho-specific immunolabeling of the ~34 kDa CDC2 protein phosphorylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15 stained with ARG52239 anti-CDK1 / CDC2 phospho (Tyr15) antibody.
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