anti-Cellubrevin antibody [4G9]
产品描述 | Mouse Monoclonal antibody [4G9] recognizes Cellubrevin |
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反应物种 | Hu, Ms |
应用 | FACS, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 4G9 |
同位型 | IgG2b, kappa |
靶点名称 | Cellubrevin |
抗原物种 | Human |
抗原 | Recombinant fragment around aa. 1-77 of Human Cellubrevin. |
偶联标记 | Un-conjugated |
別名 | Synaptobrevin-3; Vesicle-associated membrane protein 3; Cellubrevin; CEB; VAMP-3 |
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # P63024 Mouse Vesicle-associated membrane protein 3 Swiss-port # Q15836 Human Vesicle-associated membrane protein 3 |
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基因名称 | VAMP3 |
全名 | vesicle-associated membrane protein 3 |
背景介绍 | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008] |
生物功能 | SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. [UniProt] |
预测分子量 | 11 kDa |
ARG56969 anti-Cellubrevin antibody [4G9] FACS image
Flow Cytometry: HeLa cell line stained with ARG56969 anti-Cellubrevin antibody [4G9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG56969 anti-Cellubrevin antibody [4G9] WB image
Western blot: 40 µg of 1) 293T cell lysate, 2) Cellubrevin transfected 293T cell lysate stained with ARG56969 anti-Cellubrevin antibody [4G9] at 1:1000.
ARG56969 anti-Cellubrevin antibody [4G9] WB image
Western blot: 40 µg of Mouse brain tissue lysate stained with ARG56969 anti-Cellubrevin antibody [4G9] at 1:1000.
