anti-CGI58 / ABHD5 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes CGI58 / ABHD5
  • 反应物种: Hu, Ms
  • 预测物种: Dog, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 特异性: This antibody is not expected to cross-react with ABHD4.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CGI58 / ABHD5
  • Brand:
CAT.NO. : ARG64156
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes CGI58 / ABHD5
反应物种Hu, Ms
预测物种Dog, Rat
应用ICC/IF, IHC-P, WB
特异性This antibody is not expected to cross-react with ABHD4.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称CGI58 / ABHD5
抗原物种Human
抗原C-FPERPDLADQDR
偶联标记Un-conjugated
別名Lipid droplet-binding protein CGI-58; CGI58; 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; NCIE2; EC 2.3.1.51; CDS; IECN2; Abhydrolase domain-containing protein 5
应用说明
应用建议
应用推荐稀释比
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
WB0.2 - 0.6 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 51099 Human ABHD5

GeneID: 67469 Mouse ABHD5

Swiss-port # Q8WTS1 Human 1-acylglycerol-3-phosphate O-acyltransferase ABHD5

Swiss-port # Q9DBL9 Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5

背景介绍The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008]
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量39 kDa
检测图片 (4)
  • ARG64156 anti-CGI58 / ABHD5 antibody WB image

    Western Blot: NIH3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64156 anti-CGI58 / ABHD5 antibody at 0.2 µg/ml dilution.

  • ARG64156 anti-CGI58 / ABHD5 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64156 anti-CGI58 / ABHD5 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.

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