Anti-cGMP Rabbit Monoclonal Antibody [Clone RM467], 31-1359-00

Key features and details

  • Uniprot: N/A
  • Gene ID: N/A
  • Host Species: Rabbit
  • Species Reactivity: N/A
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1359-00
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Product Details
Product Details
Product NameAnti-cGMP Rabbit Monoclonal Antibody, Clone RM467
Product DescriptionRabbit monoclonal to cGMP (Cyclic Guanosine Monophosphate)
Catalog No.31-1359-00
Clone NameRM467
Product Data SheetAnti-cGMP Rabbit Monoclonal Antibody Data Sheet RM467
SpecificityRM467 reacts to cGMP (Cyclic Guanosine Monophosphate). No cross reactivity with other cyclic nucleotide or nucleoside phosphate.
ImmunogenKLH-conjugated cGMP
ApplicationELISA
ConjugateNone
Intended UseResearch Use Only
Related Product Antigen for Competitive Assay RM-PA467 (71-1359-00) - cGMP-HRP
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50µg
Concentration 1 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.05µg/mL – 0.5µg/mL
Keywords
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Product Images

Competitive ELISA of cGMP using anti-cGMP (RM467). The 96-well plate was coated
with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/ml of anti-cGMP (RM467)
(50 µL/well) was added and incubated. After wash and block, different concentrations of
cGMP (25 µL/well) were added along with 25 µL/well of 1/5,000 diluted HRP conjugated cGMP
(RM-PA467 (71-1359-00)).TMB was used to develop the color after incubation and wash.

Competitive ELISA showing the specificity of anti-cGMP (RM467). The 96-well
plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/ml
of anti-cGMP (RM467) (50 µL/well) was added and incubated. After wash
and block, cGMP and other cyclic nucleotides or nucleoside phosphates
(25 µL/well) were added along with 25 µL/well of 1/5,000 diluted HRP
conjugated cGMP (RM-PA467 (71-1359-00)).TMB was used to develop
the color after incubation and wash.

Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with
1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL of anti-cGMP (RM467)
(50 µL/well) was added and incubated.After wash and block, HeLa cell lysate
(~105 cell/mL) or control (lysate buffer only) (25 µL/well) were added along with
25 µL/well of 1/5,000 diluted HRP conjugated cGMP (RM-PA467 (71-1359-00)).
TMB was used to develop the color after incubation and wash.

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