anti-CKB / Brain Creatine Kinase antibody [8E4]
![anti-CKB / Brain Creatine Kinase antibody [8E4]](/upload/image/products/ARG57168_WB_1_210_205.jpg)
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Key features and details
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- 克隆:
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- 靶点名称:
- 抗原物种:
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CAT.NO. : ARG57168
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [8E4] recognizes CKB / Brain Creatine Kinase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 8E4 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | CKB / Brain Creatine Kinase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-381 of Human CKB |
| 偶联标记 | Un-conjugated |
| 別名 | Creatine kinase B-type; EC 2.7.3.2; BCK; B-CK; CKBB; Creatine kinase B chain; HEL-S-29; HEL-211 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CKB |
| 全名 | creatine kinase, brain |
| 背景介绍 | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt] |
| 预测分子量 | 43 kDa |
检测图片 (2)
ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] FACS image
Flow Cytometry: HeLa cell line stained with ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] WB image
Western blot: 40 µg of 1) HeLa, 2) 293T, 3) K562, and 4) LnCap cell lysates stained with ARG57168 anti-CKB / Brain Creatine Kinase antibody [8E4] at 1:1000.
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