anti-CKM / Creatine Kinase M antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes CKM / Creatine Kinase M |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 预测物种 | Cow, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CKM / Creatine Kinase M |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from the internal region of Human CKM / Creatine phosphokinase M type (NP_001815.2). (C-QKIEEIFKKAGHP) |
| 偶联标记 | Un-conjugated |
| 別名 | CKMM; M-CK; Creatine kinase M chain; EC 2.7.3.2; Creatine kinase M-type |
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 45 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CKM |
| 全名 | creatine kinase, muscle |
| 背景介绍 | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt] |
| 预测分子量 | 43 kDa |
ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35 µg of Human heart and Mouse heart lysates (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.03 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35 µg of Human (A) Pig (B) and Rat (C) heart lysates (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.3 µg/ml (A, B) and 0.1 µg/ml (C) dilutions and incubated at RT for 1 hour.
ARG58438 anti-CKM / Creatine Kinase M antibody WB image
Western blot: 35µg of Pig heart lysate (in RIPA buffer) stained with ARG58438 anti-CKM / Creatine Kinase M antibody at 0.3 µg/ml dilution. Primary incubation was 1 hour. Detected by chemiluminescence.
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