anti-Claudin 5 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Claudin 5
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Claudin 5
  • 抗原物种: Human
  • 抗原: Synthetic peptide between aa. 169-218 of Human Claudin 5.
  • Brand:
CAT.NO. : ARG66852
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Claudin 5
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Claudin 5
抗原物种Human
抗原Synthetic peptide between aa. 169-218 of Human Claudin 5.
偶联标记Un-conjugated
別名Claudin-5; TMVCF; CPETRL1; BEC1; TMDVCF; Transmembrane protein deleted in VCFS; AWAL
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:200
WB1:200 - 1:500
应用说明IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照A549
实际分子量~ 23 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol and 0.5% BSA
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12741 Mouse CLDN5

GeneID: 65131 Rat CLDN5

GeneID: 7122 Human CLDN5

基因名称CLDN5
全名claudin 5
背景介绍This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
生物功能Plays a major role in tight junction-specific obliteration of the intercellular space. [UniProt]
细胞定位Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. [UniProt]
预测分子量23 kDa
检测图片 (3)
  • ARG66852 anti-Claudin 5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung tissue stained with ARG66852 anti-Claudin 5 antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG66852 anti-Claudin 5 antibody WB image

    Western blot: A549 cell lysate stained with ARG66852 anti-Claudin 5 antibody at 1:500 dilution.

  • ARG66852 anti-Claudin 5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG66852 anti-Claudin 5 antibody at 1:200 dilution, overnight at 4°C. Negative control was used by secondary antibody only.

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