anti-CLIC4 antibody
产品描述 | Goat Polyclonal antibody recognizes CLIC4 |
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反应物种 | Hu, Ms |
预测物种 | Dog, Rat |
应用 | ICC/IF, IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | CLIC4 |
抗原物种 | Human |
抗原 | NGLKEEDKEPLIE-C |
偶联标记 | Un-conjugated |
別名 | Chloride intracellular channel protein 4; MTCLIC; H1; Intracellular chloride ion channel protein p64H1; huH1; p64H1; CLIC4L |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # Q9QYB1 Mouse Chloride intracellular channel protein 4 Swiss-port # Q9Y696 Human Chloride intracellular channel protein 4 |
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背景介绍 | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
预测分子量 | 29 kDa |
ARG64264 anti-CLIC4 antibody WB image
Western Blot: human kidney lysate (35 µg protein in RIPA buffer) stained with ARG64264 anti-CLIC4 antibody at 0.1 µg/ml dilution.
ARG64264 anti-CLIC4 antibody ICC/IF image
Immunofluorescence: MIN6 cells transiently expressing Mouse Clic4. Cells were stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution. Hoechst (blue) for nuclear staining.
ARG64264 anti-CLIC4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution followed by AP-staining.
