anti-CLIP1 / CLIP170 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG55450
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CLIP1 / CLIP170 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Chk, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CLIP1 / CLIP170 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 228-254 (N-terminus) of Human CLIP1. |
| 偶联标记 | Un-conjugated |
| 別名 | CLIP170; RSN; CLIP; CLIP-170; Restin; Cytoplasmic linker protein 1; CAP-Gly domain-containing linker protein 1; CYLN1; Cytoplasmic linker protein 170 alpha-2; Reed-Sternberg intermediate filament-associated protein |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P30622 Human CAP-Gly domain-containing linker protein 1 Swiss-port # Q922J3 Mouse CAP-Gly domain-containing linker protein 1 |
|---|---|
| 基因名称 | CLIP1 |
| 全名 | CAP-GLY domain containing linker protein 1 |
| 背景介绍 | The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 生物功能 | Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle. Note=Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 162 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules (PubMed:12231510). |
检测图片 (3)
ARG55450 anti-CLIP1 / CLIP170 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse brain tissue stained with ARG55450 anti-CLIP1 / CLIP170 antibody.
ARG55450 anti-CLIP1 / CLIP170 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55450 anti-CLIP1 / CLIP170 antibody.
ARG55450 anti-CLIP1 / CLIP170 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG55450 anti-CLIP1 / CLIP170 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
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