anti-Cofilin phospho (Ser3) antibody
Key features and details
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- 应用:
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- 宿主:
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- 抗原:
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CAT.NO. : ARG20528
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Cofilin phospho (Ser3) |
|---|---|
| 反应物种 | Hu, Ms, Rat, Chk |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | This antibody was cross-adsorbed to unphosphorylated Coflin 1 peptide then affinity purified using phospho-Cofilin 1 (Ser-3) peptide (without carrier). The antibody detects a ~19 kDa protein corresponding to the molecular mass of phosphorylated Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 靶点名称 | Cofilin |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Ser3 in human Cofilin 1 protein. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2. |
| 偶联标记 | Un-conjugated |
| 別名 | CFL; 18 kDa phosphoprotein; Cofilin, non-muscle isoform; HEL-S-15; p18; cofilin; Cofilin-1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 50% Glycerol, 1 mg/ml BSA, and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol, 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CFL1 |
| 全名 | cofilin 1 (non-muscle) |
| 背景介绍 | Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (Cofilin 2), non-muscle cofilin (Cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity. |
| 生物功能 | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2. |
检测图片 (1)
ARG20528 anti-Cofilin phospho (Ser3) antibody WB image
Western blot: Jurkat cells 1) untreated or 2,3) treated with lambda phosphatase, 3) the phosphatase was inhibited with phospho-Cofilin 1 (Ser3) peptide. The blots were stained with ARG20528 anti-Cofilin phospho (Ser3) antibody.
参考文献
MiR-128-3p suppresses breast cancer cellular progression via targeting LIMK1.
WB / Human
The microRNAs miR-200b-3p and miR-429-5p target the LIMK1/CFL1 pathway to inhibit growth and motility of breastcancer cells.
WB / Human
MiR-138-5p targeting LIMK1 suppresses breast cancer cell proliferation and motility
WB / Human
MiR-519d-3p suppresses breast cancer cell growth and motility viatargeting LIM domain kinase 1.
WB / Human
miR-143-3p targeting LIM domain kinase 1 suppresses the progression of triple-negative breast cancer cells
WB / Human
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