anti-Collagen III (Cyanogen Bromide Fragments) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Collagen III (Cyanogen Bromide Fragments)
  • 反应物种: Hu, Ms, Rat
  • 应用: ELISA, FACS, ICC/IF, IHC, IP, RIA, WB
  • 特异性: The antibody reacts with collagen type III from Human, Mouse and Rat. It exhibits no cross reactivity.
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Collagen III (Cyanogen Bromide Fragments)
  • 抗原物种: Mouse
  • Brand:
CAT.NO. : ARG23681
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Collagen III (Cyanogen Bromide Fragments)
反应物种Hu, Ms, Rat
应用ELISA, FACS, ICC/IF, IHC, IP, RIA, WB
特异性The antibody reacts with collagen type III from Human, Mouse and Rat. It exhibits no cross reactivity.
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Collagen III (Cyanogen Bromide Fragments)
抗原物种Mouse
抗原Collagen type III BrCN fragment.
偶联标记Un-conjugated
別名EDS4A; Collagen alpha-1(III) chain
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC2 - 8 µg/ml
IP4 - 16 µg/ml
RIAAssay-dependent
WB150 - 600 ng/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化The IgG fraction was purified from rabbit neat serum by affinity chromatography.
缓冲液PBS
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 1281 Human COL3A1

GeneID: 12825 Mouse COL3A1

GeneID: 84032 Rat COL3A1

基因名称COL3A1
全名collagen, type III, alpha 1
背景介绍This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
生物功能Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt]
预测分子量139 kDa
翻译后修饰Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt]
检测图片 (1)
  • ARG23681 anti-Collagen III (Cyanogen Bromide Fragments) antibody WB image

    Western blot: Collagen type I, II and III with following treatments: (a) Coomassie stain after blotting, (b) Immunostain with ARG23681 anti-Collagen III (Cyanogen Bromide Fragments) antibody, (c) India ink stain of transferred proteins onto Immunobilon membranes.

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