anti-COTL1 antibody [1D6]

CAT.NO. : ARG57135

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [1D6] recognizes COTL1
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1D6
同位型	IgG2b, kappa
靶点名称	COTL1
抗原物种	Human
抗原	Recombinant fragment around aa. 1-142 of Human COTL1
偶联标记	Un-conjugated
別名	Coactosin-like protein; CLP

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	1:250 - 1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 23406 Human COTL1 Swiss-port # Q14019 Human Coactosin-like protein
基因名称	COTL1
全名	coactosin-like F-actin binding protein 1
背景介绍	This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008]
生物功能	Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis. [UniProt]
预测分子量	16 kDa

检测图片 (4)

ARG57135 anti-COTL1 antibody [1D6] FACS image
Flow Cytometry: HeLa cell line stained with ARG57135 anti-COTL1 antibody [1D6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 40 µg of SW480 cell lysate stained with ARG57135 anti-COTL1 antibody [1D6] at 1:1000.
ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 20 µg of 1) 293T, and 2) COTL1 transfected 293T cell lysate stained with ARG57135 anti-COTL1 antibody [1D6] at 1:2000.
ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 40 µg of 1) PC3, 2) A549, 3) U87-MG, and 4) HeLa cell lysates stained with ARG57135 anti-COTL1 antibody [1D6] at 1:500.

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