anti-CRB3 antibody [14F9]

Key features and details

  • 产品描述: Rat Monoclonal antibody [14F9] recognizes CRB3
  • 反应物种: Hu, Ms
  • 应用: ICC/IF, IHC, WB
  • 宿主: Rat
  • 克隆: Monoclonal
  • 克隆号: 14F9
  • 同位型: IgG2c
  • 靶点名称: CRB3
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56564
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Product Details
概述
产品描述Rat Monoclonal antibody [14F9] recognizes CRB3
反应物种Hu, Ms
应用ICC/IF, IHC, WB
宿主Rat
克隆Monoclonal
克隆号14F9
同位型IgG2c
靶点名称CRB3
抗原物种Human
抗原Full length of Human CRB3 protein.
偶联标记Un-conjugated
別名Protein crumbs homolog 3
应用说明
应用建议
应用推荐稀释比
ICC/IF1:100
IHC1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified.
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 224912 Mouse CRB3

GeneID: 92359 Human CRB3

Swiss-port # Q8QZT4 Mouse Protein crumbs homolog 3

Swiss-port # Q9BUF7 Human Protein crumbs homolog 3

基因名称CRB3
全名crumbs family member 3
背景介绍This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
生物功能Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. [UniProt]
预测分子量13 kDa
检测图片 (4)
  • ARG56564 anti-CRB3 antibody [14F9] ICC/IF image

    Immunofluorescence: Overexpressed human CRB3 in lysates of HeLa cells stained with ARG56564 anti-CRB3 antibody [14F9].

  • ARG56564 anti-CRB3 antibody [14F9] WB image

    Western blot: analysis of full length CRB3 (GST-CRB3) but not the extracellular domain of CRB3 (GST-CRB3extra). ARG56564 anti-CRB3 antibody [14F9] was used at 1:1000 dilution.

  • ARG56564 anti-CRB3 antibody [14F9] ICC/IF image

    Immunofluorescence: Endogenous human CRB3 expression in the mouse intestine stained with ARG56564 anti-CRB3 antibody [14F9]. DAPI was used to stain the nuclei in HeLa cells, whereas E-cadherin staining was used to highlight the lateral membrane in mouse intestine.

  • ARG56564 anti-CRB3 antibody [14F9] WB image

    Western blot: analysis of overexpressed (HEK293T cells) and endogenous CRB3 (mouse tissues). The blots were stained with ARG56564 anti-CRB3 antibody [14F9].

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