anti-CTGF antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CTGF
  • 反应物种: Hu, Ms
  • 预测物种: Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CTGF
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66979
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CTGF
反应物种Hu, Ms
预测物种Rat
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CTGF
抗原物种Human
抗原Synthetic peptide around the middle region of Human CTGF.
偶联标记Un-conjugated
別名Insulin-like growth factor-binding protein 8; IGFBP-8; CCN2; IBP-8; HCS24; Connective tissue growth factor; IGF-binding protein 8; CCN family member 2; IGFBP8; Hypertrophic chondrocyte-specific protein 24; NOV2
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200 - 1:300
IHC-P1:50 - 1:150
WB1:500 - 1:1000
应用说明IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HepG2; Spleen; Skin carcinoma
属性
形式Liquid
纯化Affinity purified.
缓冲液100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol.
抗菌剂0.025% ProClin 300
稳定剂20% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 14219 Mouse CTGF

GeneID: 1490 Human CTGF

Swiss-port # P29268 Mouse Connective tissue growth factor

Swiss-port # P29279 Human Connective tissue growth factor

基因名称CTGF
全名connective tissue growth factor
背景介绍The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
生物功能Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. [UniProt]
预测分子量38 kDa; 17 kDa
检测图片 (4)
  • ARG66979 anti-CTGF antibody ICC/IF image

    Immunofluorescence: HepG2 cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66979 anti-CTGF antibody (red) at 1:200 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.

  • ARG66979 anti-CTGF antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human Skin carcinoma tissue stained with ARG66979 anti-CTGF antibody at 1:100 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).

  • ARG66979 anti-CTGF antibody WB image (Human)

    Western blot: HepG2 condition medium stained with ARG66979 anti-CTGF antibody at 1:700 dilution, overnight at 4°C.

  • ARG66979 anti-CTGF antibody WB image (Mouse)

    Western blot: 50 µg of Mouse Spleen tissue cell lysate stained with ARG66979 anti-CTGF antibody at 1:700 dilution, overnight at 4°C.

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