anti-CX3CL1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CX3CL1
  • 反应物种: Hu
  • 应用: ELISA, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CX3CL1
  • 抗原物种: Human
  • 抗原: E. coli derived recombinant Human CX3CL1.(QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG)
  • Brand:
CAT.NO. : ARG66031
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CX3CL1
反应物种Hu
应用ELISA, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CX3CL1
抗原物种Human
抗原E. coli derived recombinant Human CX3CL1.
(QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG)
偶联标记Un-conjugated
別名neurotactin; CXC3; C3Xkine; NTN; NTT; C-X3-C motif chemokine 1; CXC3C; Neurotactin; ABCD-3; SCYD1; Fractalkine; Small-inducible cytokine D1; CX3C membrane-anchored chemokine; fractalkine
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66032 as a detection antibody
IHC-P0.25 µg/ml
WB0.1 - 0.2 µg/ml
应用说明IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6376 Human CX3CL1

Swiss-port # P78423 Human Fractalkine

基因名称CX3CL1
全名chemokine (C-X3-C motif) ligand 1
生物功能The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. [UniProt]
预测分子量42 kDa
翻译后修饰A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans.
检测图片 (3)
  • ARG66031 anti-CX3CL1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas malignant ductal adenocarcinoma. The recommended ARG66031 anti-CX3CL1 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG66031 anti-CX3CL1 antibody WB image

    Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66031 anti-CX3CL1 antibody, under non-reducing conditions.

  • ARG66031 anti-CX3CL1 antibody WB image

    Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66031 anti-CX3CL1 antibody, under reducing conditions.

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