anti-CXCL3 / GRO gamma antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CXCL3 / GRO gamma
  • 反应物种: Hu, Ms
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CXCL3 / GRO gamma
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human GRO gamma.(ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN)
  • Brand:
CAT.NO. : ARG56693
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CXCL3 / GRO gamma
反应物种Hu, Ms
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CXCL3 / GRO gamma
抗原物种Human
抗原E.coli derived Recombinant Human GRO gamma.
(ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN)
偶联标记Un-conjugated
別名Macrophage inflammatory protein 2-beta; MIP2-beta; SCYB3; GRO-gamma; Growth-regulated protein gamma; 5-73; GROg; 1-73; MIP2B; C-X-C motif chemokine 3; CINC-2b; GRO3; MIP-2b
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56802 as a detection antibody
IHC-P0.5 - 0.7 µg/ml
Neut1.0 - 2.0 µg/ml (To yield [ND50] of the biological activity of Human GRO - γ (100 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2921 Human CXCL3

GeneID: 330122 Mouse CXCL3

Swiss-port # P19876 Human C-X-C motif chemokine 3

Swiss-port # Q6W5C0 Mouse C-X-C motif chemokine 3

基因名称CXCL3
全名chemokine (C-X-C motif) ligand 3
背景介绍This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]
生物功能Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. [UniProt]
预测分子量11 kDa
翻译后修饰N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
检测图片 (5)
  • ARG56693 anti-CXCL3 / GRO gamma antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma. The recommended ARG56693 anti-CXCL3 / GRO gamma antibody concentration is 0.7 µg/ml - 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56693 anti-CXCL3 / GRO gamma antibody WB image

    Western blot: 250 - 0.24 ng of Human GRO gamma stained with ARG56693 anti-CXCL3 / GRO gamma antibody, under reducing conditions.

  • ARG56693 anti-CXCL3 / GRO gamma antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon/rectum adenocarcinoma. The recommended ARG56693 anti-CXCL3 / GRO gamma antibody concentration is 0.7 µg/ml - 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56693 anti-CXCL3 / GRO gamma antibody WB image

    Western blot: 250 - 0.24 ng of Human GRO gamma stained with ARG56693 anti-CXCL3 / GRO gamma antibody, under non-reducing conditions.

  • ARG56693 anti-CXCL3 / GRO gamma antibody standard curve image

    Sandwich ELISA: ARG56693 anti-CXCL3 / GRO gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56802 anti-CXCL3 / GRO gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

参考文献

Self-recruited neutrophils trigger over-activated innate immune response and phenotypic change of cardiomyocytes in fulminant viral myocarditis

Neut / Mouse

Huihui Li et al.
Cell discovery,  (2023)

publication_link

 

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