anti-Cyclin B1 antibody [V152]
![anti-Cyclin B1 antibody [V152]](/upload/image/products/ARG62456_WB_Sheet_HeLa_170203_210_205.jpg)
![anti-Cyclin B1 antibody [V152]](/upload/image/products/ARG62456_WB_Sheet_HeLa_170203.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [V152] recognizes Cyclin B1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 特异性 | Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | V152 |
| 同位型 | IgG1 |
| 靶点名称 | Cyclin B1 |
| 抗原物种 | Hamster |
| 抗原 | His-tagged Hamster Cyclin B1 expressed in bacteria |
| 偶联标记 | Un-conjugated |
| 別名 | G2/mitotic-specific cyclin-B1; CCNB |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | Ccnb1 |
| 全名 | cyclin B1 |
| 背景介绍 | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) Related products: Cyclin B1 antibodies; Cyclin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| 预测分子量 | 48 kDa |
| 翻译后修饰 | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
ARG62456 anti-Cyclin B1 antibody [V152] WB image
Western blot: 20 µl of HeLa cell lysate stained with ARG62456 anti-Cyclin B1 antibody [V152] at 1 µg/ml dilution.
克隆号文献
Casein kinase 1δ-dependent Wee1 protein degradation.
WB / Human
Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity.
WB /
Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication.
Tumor dynamics in response to antiangiogenic therapy with oral metronomic topotecan and pazopanib in neuroblastoma xenografts.
IHC-P / Mouse
The role of RING box protein 1 in mouse oocyte meiotic maturation.
APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing.
WB /
Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.
ICC/IF /
Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.
IHC / Rat
Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.
FACS / Human
Citron kinase is a cell cycle-dependent, nuclear protein required for G2/M transition of hepatocytes.
WB / Rat
Kinetics of endomitosis in primary murine megakaryocytes.
FACS / Mouse
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