anti-CYP19A1 / Aromatase antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes CYP19A1 / Aromatase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CYP19A1 / Aromatase |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to Y241-H503 of Human CYP19A1 / Aromatase. |
| 偶联标记 | Un-conjugated |
| 別名 | ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO |
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 58 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CYP19A1 |
| 全名 | cytochrome P450, family 19, subfamily A, polypeptide 1 |
| 背景介绍 | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| 生物功能 | Catalyzes the formation of aromatic C18 estrogens from C19 androgens. [UniProt] |
| 细胞定位 | Membrane; Peripheral membrane protein. [UniProt] |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1). [UniProt] |
ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
ARG41572 anti-CYP19A1 / Aromatase antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta lysate stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 0.5 µg/ml dilution, overnight at 4°C.
ARG41572 anti-CYP19A1 / Aromatase antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal Goat serum and then stained with ARG41572 anti-CYP19A1 / Aromatase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
ARG41572 anti-CYP19A1 / Aromatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41572 anti-CYP19A1 / Aromatase antibody at 1 µg/ml dilution, overnight at 4°C.
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