anti-Cytokeratin 7 antibody [K72.7]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [K72.7] recognizes Cytokeratin 7
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: K72.7
  • 同位型: IgG1, kappa
  • 靶点名称: Cytokeratin 7
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56043
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Product Details
概述
产品描述Mouse Monoclonal antibody [K72.7] recognizes Cytokeratin 7
反应物种Hu
应用FACS, ICC/IF, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号K72.7
同位型IgG1, kappa
靶点名称Cytokeratin 7
抗原物种Human
抗原A semi-purified cytokeratin preparation.
偶联标记Un-conjugated
別名SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7
应用说明
应用建议
应用推荐稀释比
FACS0.5 - 1 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB0.5 - 2 µg/ml
应用说明IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HeLa
实际分子量~ 55 kDa
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂0.05% Sodium azide
稳定剂0.1 mg/ml BSA
浓度0.2 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3855 Human KRT7

Swiss-port # P08729 Human Keratin, type II cytoskeletal 7

基因名称KRT7
全名keratin 7, type II
背景介绍Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
生物功能Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt]
细胞定位Cytoplasmic
研究领域CK7/CK20 Carcinoma Study antibody
预测分子量51 kDa
翻译后修饰Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
检测图片 (5)
  • ARG56043 anti-Cytokeratin 7 antibody [K72.7] ICC/IF image

    Immunofluorescence: Methanol-fixed HeLa cells stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7] (green). Reddot (red) for nuclear staining.

  • ARG56043 anti-Cytokeratin 7 antibody [K72.7] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung SCC stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].

  • ARG56043 anti-Cytokeratin 7 antibody [K72.7] WB image

    Western blot: HeLa cell lysate stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].

  • ARG56043 anti-Cytokeratin 7 antibody [K72.7] FACS image

    Flow Cytometry: PFA-fixed HeLa cells stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7] (blue); Isotype control (red).

  • ARG56043 anti-Cytokeratin 7 antibody [K72.7] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded ovarian carcinoma stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].

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