anti-DCTN1 / p150-glued antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40795
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes DCTN1 / p150-glued |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DCTN1 / p150-glued |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 945-1139 of Human DCTN1 (NP_001128513.1). |
| 偶联标记 | Un-conjugated |
| 別名 | P135; p150-glued; p135; DP-150; Dynactin subunit 1; 150 kDa dynein-associated polypeptide; DAP-150 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | U-251MG |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DCTN1 |
| 全名 | dynactin 1 |
| 背景介绍 | This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008] |
| 生物功能 | Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, spindle, cell cortex. Nucleus envelope. [UniProt] |
| 预测分子量 | 142 kDa |
| 翻译后修饰 | Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase. [UniProt] |
检测图片 (2)
ARG40795 anti-DCTN1 / p150-glued antibody WB image
Western blot: 25 µg of U-251MG cell lysate stained with ARG40795 anti-DCTN1 / p150-glued antibody at 1:1000 dilution.
ARG40795 anti-DCTN1 / p150-glued antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells were immunoprecipitated and stained with ARG40795 anti-DCTN1 / p150-glued antibody at 1:1000 dilution.
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