anti-DDAH2 antibody
产品描述 | Goat Polyclonal antibody recognizes DDAH2 |
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反应物种 | Hu |
预测物种 | Ms, Rat, Cow, Dog |
应用 | FACS, ICC/IF, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | DDAH2 |
抗原物种 | Human |
抗原 | SSLCLVLSTRPHS |
偶联标记 | Un-conjugated |
別名 | Dimethylarginine dimethylaminohydrolase 2; S-phase protein; G; DDAH; NG30; G6a; EC 3.5.3.18; N; Dimethylargininase-2; HEL-S-277; DDAH-2; Protein G6a; DDAHII |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # O95865 Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 |
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背景介绍 | This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008] |
研究领域 | Neuroscience antibody |
预测分子量 | 30 kDa |
ARG63168 anti-DDAH2 antibody WB image
Western Blot: Human Lung lysate (35 µg protein in RIPA buffer) stained with ARG63168 anti-DDAH2 antibody at 0.1 µg/ml dilution.
ARG63168 anti-DDAH2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63168 anti-DDAH2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63168 anti-DDAH2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG63168 anti-DDAH2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
