anti-DDDDK tag antibody [SQab1880]

Key features and details

产品描述: Recombinant Rabbit Monoclonal antibody [SQab1880] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies). The antibody recognizes the hydrophilic epitope tagging peptide sequence DDDDK (Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.
反应物种: Other
应用: FACS, ICC/IF, IP, WB
宿主: Rabbit
克隆: Monoclonal
克隆号: SQab1880
同位型: IgG
靶点名称: DDDDK tag
抗原物种: Others

Brand:

CAT.NO. : ARG66332

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Size:

100 μl 50 μl 20μl

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab1880] recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies). The antibody recognizes the hydrophilic epitope tagging peptide sequence DDDDK (Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins.
反应物种	Other
应用	FACS, ICC/IF, IP, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab1880
同位型	IgG
靶点名称	DDDDK tag
抗原物种	Others
抗原	KLH-conjugated DYKDDDDK.
偶联标记	Un-conjugated

应用说明

应用建议

应用	推荐稀释比
FACS	1:1000 - 1:2000
ICC/IF	1:1500 - 1:10000
IP	1:50
WB	1:10000 - 1:20000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

研究领域	Controls and Markers antibody; Tag Internal Control antibody

检测图片 (5)

ARG66332 anti-DDDDK tag antibody [SQab1880] ICC/IF image
Immunofluorescence: 293 cells trasnfected with C-terminal DYKDDDDK tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with DAPI (left) or ARG66332 anti-DDDDK tag antibody [SQab1880] at 1:10000 and 4°C (right).
ARG66332 anti-DDDDK tag antibody [SQab1880] FACS image
Flow Cytometry: 293 cells transfected with N-terminal DYKDDDDK tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66332 anti-DDDDK tag antibody [SQab1880] (light blue histogram) at 1:2000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (white histogram) was used as a control.
ARG66332 anti-DDDDK tag antibody [SQab1880] WB image
Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 µg of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with N-terminal DYKDDDDK tagged gene (ARG66332 at 1:10000, right).
ARG66332 anti-DDDDK tag antibody [SQab1880] IP image
Immunoprecipitation: 0.1 mg of 293 whole cells lysate transfected with N-terminal His tagged gene immunoprecipitated (1:50) and stained with ARG66332 anti-DDDDK tag antibody [SQab1880]. 1) PBS (Control) and 2) Primary antibody.
ARG66332 anti-DDDDK tag antibody [SQab1880] WB image
Western blot: Testing of ARG66332 anti-DDDDK tag antibody [SQab1880]. 3 µg of 293 cell lysate without any transfection (ARG66332 at 1:2000, left) or transfected with C-terminal DYKDDDDK tagged gene (ARG66332 at 1:100000, right).