anti-DGAT1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes DGAT1
  • 反应物种: Hu, Ms
  • 预测物种: Cow, Rat, Dog
  • 应用: FACS, IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: DGAT1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG64119
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes DGAT1
反应物种Hu, Ms
预测物种Cow, Rat, Dog
应用FACS, IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称DGAT1
抗原物种Human
抗原C-QNSMKPFKDMDYS
偶联标记Un-conjugated
別名ARGP1; Diacylglycerol O-acyltransferase 1; ARAT; Retinol O-fatty-acyltransferase; DGAT; EC 2.3.1.76; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; Diglyceride acyltransferase; DIAR7; EC 2.3.1.20
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
IHC-P3 - 5 µg/ml
WB0.01 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 13350 Mouse DGAT1

GeneID: 8694 Human DGAT1

Swiss-port # O75907 Human Diacylglycerol O-acyltransferase 1

Swiss-port # Q9Z2A7 Mouse Diacylglycerol O-acyltransferase 1

背景介绍The enzyme encoded by this gene utilizes diacylglycerol and fatty acyl CoA as substrates in order to catalyze the final stage of triacylglycerol synthesis. It is also involved in cellular as well as physiological metabolic processes. [provided by RefSeq, Jul 2008]
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量55 kDa
检测图片 (3)
  • ARG64119 anti-DGAT1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64119 anti-DGAT1 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG64119 anti-DGAT1 antibody WB image

    Western blot: 35 µg of Mouse liver lysate (in RIPA buffer) stained with ARG64119 anti-DGAT1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.

  • ARG64119 anti-DGAT1 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG64119 anti-DGAT1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

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