Anti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody [Clone RM168]; H3K18me2; Histone H3 (dimethyl K18); 31-1075-00; 31-1075-00-S

Key features and details

  • Uniprot: P84243
  • Gene ID: 8350
  • Host Species: Rabbit
  • Species Reactivity: Human Vertebrates
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1075-00
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Product Details
Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM168
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 18, H3K18me2, Histone H3 (dimethyl K18)
Catalog No.31-1075-00
Clone NameRM168
Product Data SheetAnti-Dimethyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody Data Sheet RM168
SpecificityRM168 reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18, monomethylated Lysine 18 (K18me1), or other methylations in histone H3.
ImmunogenA dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys18).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 µg/mL – 1 µg/mL;
ELISA: 0.1 µg/mL - 0.5 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
A

A Peptide dotblot shows RM168 only reacts to Histone.H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).

RM168

RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 µg/mL, showed a band of histone H3dimethylated at Lysine 18 (K18me2) in HeLa cells.

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