Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM135]; H3K4me2; Histone H3 (dimethyl K4); ChIP Validated; 31-1037-00; 31-1037-00-S
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![Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM135]; H3K4me2; Histone H3 (dimethyl K4); ChIP Validated; 31-1037-00; 31-1037-00-S](/upload/image/products/rm135_1.png)
![Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM135]; H3K4me2; Histone H3 (dimethyl K4); ChIP Validated; 31-1037-00; 31-1037-00-S](/upload/image/products/Fig3_RM135_ICC.png)
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CAT.NO. : 31-1037-00
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Product Details
Product Details
| Product Name | Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135 |
| Product Description | Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4) |
| Catalog No. | 31-1037-00 |
| Clone Name | RM135 |
| Product Data Sheet | Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM135 |
| Specificity | RM135 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3. |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4). |
| Application | Chromatin IP (ChIP) Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex Immunohistochemistry (IHC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ChIP: 1 µg - 5 µg; Western Blot: 0.02 µg/mL – 0.2 µg/mL; ICC: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. IHC: 1 µg/mL - 2 µg/mL. |
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Product Images
 | RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 µg/mL, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Stomach tissue sections, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135. |
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