Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM181]; H3K79me2; Histone H3 (dimethyl K79); 31-1065-00; 31-1065-00-S
![Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM181]; H3K79me2; Histone H3 (dimethyl K79); 31-1065-00; 31-1065-00-S](/upload/image/products/RM181-Fig2.png)
![Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM181]; H3K79me2; Histone H3 (dimethyl K79); 31-1065-00; 31-1065-00-S](/upload/image/products/RM181-Fig1_E.png)
![Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM181]; H3K79me2; Histone H3 (dimethyl K79); 31-1065-00; 31-1065-00-S](/upload/image/products/RM181-Fig3_e.png)
![Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM181]; H3K79me2; Histone H3 (dimethyl K79); 31-1065-00; 31-1065-00-S](/upload/image/products/HepG2_H3K79M2_IHC.png)
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CAT.NO. : 31-1065-00
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Product Details
Product Details
| Product Name | Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181 |
| Product Description | Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79) |
| Catalog No. | 31-1065-00 |
| Clone Name | RM181 |
| Product Data Sheet | Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181 |
| Specificity | RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3. |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79). |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunohistochemistry (IHC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.25 µg/mL – 1 µg/mL; IHC:0.1 µg/mL – 1 µg/mL; ChIP: 2 µg/mL - 10 µg.mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. |
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Product Images
 | RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 µg/mL, showed a band of histone H3dimethylated at Lysine 79 (K79me2) in HeLa cells. |
 | ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunohistochemistry staining of HepG2 cells using Anti-Dimethyl-Histone H3 (Lys79) antibody, Clone RM181. |
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