Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM322], 31-1207-00
![Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM322], 31-1207-00](/upload/image/products/Fig2_H3K9m2K14ac_WB_e.png)
![Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM322], 31-1207-00](/upload/image/products/Fig1_H3K9m2K14ac_Luminex_e.png)
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CAT.NO. : 31-1207-00
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Product Details
Product Details
| Product Name | Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM322 |
| Product Description | Rabbit monoclonal to Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) |
| Catalog No. | 31-1207-00 |
| Clone Name | RM322 |
| Product Data Sheet | Anti-Dimethyl-Histone H3 (Lys9) and Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody Data Sheet RM322 |
| Specificity | RM322 reacts to Histone H3 only when modified by both methylation at lysine 9 and acetylation at lysine 14 (K9me2/K14ac). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), trimethylated Lysine 9 (K9me3), or acetylated Lysine 9/14 only, in histone H3. |
| Immunogen | A dimethyl-peptide corresponding to dimethyl-Histone H3 (Lys9) and an acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) |
| Application | Western Blot (WB) Multiplex |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | WB: 0.1 µg/mL – 1 µg/mL; Multiplex: 0.01 µg/mL – 1 µg/mL. |
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Product Images
 | RM322 specifically reacts to Histone H3 only when modified at both dimethyl-lysine 9 and acetyl-lysine 14 (K9me2/K14ac). No cross reactivity with non-modified Lysine 9/14, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, acetylated Lysine 9/14 ONLY, or methylated Lysine 14 (K14me2) ONLY, in Histone H3. |
 | Western Blot of HeLa cells (1) and recombinant histone H3.3 (2) using RM322 at 0.5 µg/mL. |
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