Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM151]; H3K9me2; Histone H3 (dimethyl K9); 31-1059-00; 31-1059-00-S
![Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM151]; H3K9me2; Histone H3 (dimethyl K9); 31-1059-00; 31-1059-00-S](/upload/image/products/RM151-Fig2_e.png)
![Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM151]; H3K9me2; Histone H3 (dimethyl K9); 31-1059-00; 31-1059-00-S](/upload/image/products/RM151-Fig1_e.png)
![Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM151]; H3K9me2; Histone H3 (dimethyl K9); 31-1059-00; 31-1059-00-S](/upload/image/products/RM151-Fig3_e.png)
![Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [Clone RM151]; H3K9me2; Histone H3 (dimethyl K9); 31-1059-00; 31-1059-00-S](/upload/image/products/Fig4_RM151_ICC_e.png)
Key features and details
- Uniprot:
- Gene ID:
- Host Species:
- Species Reactivity:
- Concentration:
-
Brand:
Product Details
| Product Name | Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151 |
| Product Description | Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9) |
| Catalog No. | 31-1059-00 |
| Clone Name | RM151 |
| Product Data Sheet | Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151 |
| Specificity | RM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3. |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9). |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex Immunohistochemistry (IHC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt. |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.25 µg/mL – 1 µg/mL; ChIP: 2 µg/mL - 10 µg/mL; ICC: 0.5 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. IHC: 0.1 µg/mL – 0.2 µg/mL |
| References for Dimethyl-Histone H3 (Lys9) antibody [RM151] | Xiong H et al. Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss. J Assoc Res Otolaryngol. (2019), 10.1007/s10162-019-00714-6. ICC, IHC; Human. Read more (PubMed: 30710318) |
| . | |
 | RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 µg/mL, showed a band of histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells. |
 | ChIP performed on HeLa cells using H3K9me2 antibody (RM151, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Dimethyl-Histone H3 (Lys9) Rabbit mAb RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Stomach tissue sections, using Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151 |
New Products
