anti-DLC1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes DLC1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Pig |
| 应用 | ICC/IF, IHC-P |
| 特异性 | This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DLC1 |
| 抗原物种 | Human |
| 抗原 | SVAIRKRSWEEHC |
| 偶联标记 | Un-conjugated |
| 別名 | STARD12; Rho GTPase-activating protein 7; p122-RhoGAP; START domain-containing protein 12; HP; StARD12; StAR-related lipid transfer protein 12; Rho-type GTPase-activating protein 7; DLC-1; Deleted in liver cancer 1 protein; ARHGAP7; HP protein |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 171 kDa |
ARG63634 anti-DLC1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG63634 anti-DLC1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63634 anti-DLC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse adrenal gland tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63634 anti-DLC1 antibody at 4 µg/ml dilution followed by HRP-staining.
ARG63634 anti-DLC1antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Adrenal Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63634 anti-DLC1 (Isoforms 1 and 3) antibody at 5 µg/ml dilution followed by AP-staining.
ARG63634 anti-DLC1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63634 anti-DLC1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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