anti-DMP1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes DMP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_004398.1; NP_001073380.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | DMP1 |
| 抗原物种 | Human |
| 抗原 | C-ENSEQANTQDSG |
| 偶联标记 | Un-conjugated |
| 別名 | DMP-1; Dentin matrix protein 1; ARHP; Dentin matrix acidic phosphoprotein 1; ARHR |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # Q13316 Human Dentin matrix acidic phosphoprotein 1 |
|---|---|
| 背景介绍 | Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 56 kDa |
| 翻译后修饰 | Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. |
ARG64688 anti-NGEF Antibody WB image
Western blot: human dental pulp stem cells (hDPSCs) stained with ARG64688 anti-NGEF Antibody.
From Kim Hong-Bae et al. Sci Rep. (2018), doi: 10.1038/s41598-018-19395-x, Fig. 5C.
ARG64688 anti-DMP1 antibody ICC/IF image
Immunofluorescence: WJMSC cells fixed with 10% Formalin for 20 min and blocked with PBS, 5% FBS and 0.1% Triton at RT for 30 min. Cells were incubated in PBS with 5% FBS and stained with ARG64688 anti-DMP1 antibody (green) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG64688 anti-DMP1 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG64688 anti-DMP1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
Development of hybrid scaffolds with biodegradable polymer composites and bioactive hydrogels for bone tissue engineering
ICC/IF / Human
Pulse Frequency Dependency of Photobiomodulation on Osteogenic Differentiation of Human Dental Pulp Stem Cells.
WB / Human
Effects of pulsing of light on the dentinogenesis of dental pulp stem cells in vitro.
WB / Human
New Products
