anti-DMPO Nitrone Adduct antibody [N166A4]
![anti-DMPO Nitrone Adduct antibody [N166A4]](/upload/image/products/ARG20538_WB_1_210_205.jpg)
![anti-DMPO Nitrone Adduct antibody [N166A4]](/upload/image/products/ARG20538_IF_1.jpg)
![anti-DMPO Nitrone Adduct antibody [N166A4]](/upload/image/products/ARG20538_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
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- 同位型:
- 靶点名称:
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CAT.NO. : ARG20538
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [N1664A] recognizes DMPO Nitrone Adduct |
|---|---|
| 反应物种 | Other |
| 应用 | ELISA, ICC/IF, IHC, IP, WB |
| 特异性 | Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | N1664A |
| 同位型 | IgG1 |
| 靶点名称 | DMPO Nitrone Adduct |
| 抗原 | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems.Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals. |
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| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
检测图片 (2)
ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] ICC/IF image
Immunofluorescence: Mouse macrophage cell lines stained with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] at 10 ug/ml dilution.
ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4] WB image
Western blot: 1) 10 uM Hb (Hemoglobin), 2) 10 uM Hb + 100 uM HOCI, 3) 10 uM Hb + 500 uM HOCI, 4) 10 uM Hb + 1000 uM HOCI, 5)-8) as above with 20 mM DMPO, 9)-12) as above with 100 mM DMPO. Staining with ARG20538 anti-DMPO Nitrone Adduct antibody [N166A4].
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