anti-eEF1D antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes eEF1D
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Mk
  • 应用: FACS, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: eEF1D
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG58602
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes eEF1D
反应物种Hu
预测物种Ms, Rat, Mk
应用FACS, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称eEF1D
抗原物种Human
抗原KLH-conjugated synthetic peptide between aa. 1-30 of Human eEF1D.
偶联标记Un-conjugated
別名Antigen NY-CO-4; FP1047; EF-1-delta; EF1D; EF-1D; Elongation factor 1-delta
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Ramos
属性
形式Liquid
纯化This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液PBS and 0.09% (W/V) Sodium azide.
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 1936 Human EEF1D

Swiss-port # P29692 Human Elongation factor 1-delta

基因名称EEF1D
全名eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
背景介绍This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]
生物功能Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). [UniProt]
预测分子量31 kDa
检测图片 (2)
  • ARG58602 anti-eEF1D antibody WB image

    Western blot: 35 µg of Ramos cell lysate stained with ARG58602 anti-eEF1D antibody.

  • ARG58602 anti-eEF1D antibody FACS image

    Flow Cytometry: Ramos cells stained with ARG58602 anti-eEF1D antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.

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